Effects of aqueous leaf extract of Telfairia occidentalis on haematological parameters and liver enzymes in male Wistar rats

  • IO Osonuga
  • AS Faponle Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Sagamu Campus, Sagamu, Ogun State
  • EN Ezima
  • TK Adenowo
  • AA Adelegan
Keywords: Haematological indices, Liver enzymes, Red Blood Cells, Telfairia occidentalis


Background: The leaves of Telfairia occidentalis (locally known as Ugu) are widely consumed as part of a staple in the southern region of Nigeria. Its nutritional benefits include its rich mineral contents and antioxidant properties. It has been suggested that the leaf extracts may affect blood parameters.

Objectives: To investigate the effects of aqueous extracts of T. occidentalis leaves on haematological indices and liver enzymes in rats.

Methods: Twenty-four male Wistar rats weighing between 150g and 200g were used for the study. They were categorized into four groups of six rats each viz: high-dose, medium-dose, low-dose, and control groups. The leaf extract was administered in doses of 300mg/kg, 200mg/kg, and 100 mg/kg, respectively, while the control group received distilled water rather than leaf extracts. 

Results: There was a dose-dependent decrease in the concentrations of liver enzymes and an increase in blood parameters. There was a significant difference (p = 0.000) in the mean red blood cells countof the control group (7.5±0.2×1012/L) compared to the low-dose group (9.1±0.1×1012/L), the medium-dose group (11.7±0.2×1012/L) and the high-dose group (13.3±0.2×1012/L).For the liver enzymes, there was a significant decrease in the mean AST levels in the high-dose group (42.8±3.5 IU/L), the medium-dose group (53.7±5.7IU/L) and the low-dose group (68.5±3.5IU/L) were compared to the value for the control group (88.6 ±2.5× 1012/L).

Conclusions: Using an animal model, Telfairia occidentalis may have hepatoprotective and haemopoietic properties.


Shah JS, Shah MB, Goswami SS, Santani DD. Mechanism of action of antiulcer activity of Manilkarahexandra against experimentally induced gastric ulcers in rats. Pharmacog Mag 2006; 5(2): 40-45.

Sofowora A. Medicinal plants and traditional medicine in Africa. 2nd Edition. Ibadan, Nigeria: Spectrum Books; 1996.

Badifu GI, Ogunsua AO. Chemical composition of kernels from some species of Cucurbitaceae grown in Nigerian plant foods. Hum Nutr 1991; 41:35–44.

Longe OG, Farinu GO, Fetuga BL. Nutritional value of the fluted pumpkin (Telfairiaoccidentalis). J Agric Food Chem 1983; 311: 989-992.

Odoemena CS Onyeneke EC. Lipids of fluted pumpkin Telfairiaoccidentalis. First Afr Conf Biochem of Lipids 1998; 147-151.

FasuyiAO, Nonyerem AD. Biochemical, nutritional and haematological implications of Telfairiaoccidentalis leaf meal as protein supplement in broiler starter diets. Afr J Biotechnol 2007; 6: 1055-1063.

Kayode AAA, Kayode OT, and Odetola AA. Telfairiaoccidentalis ameliorates oxidative brain damage in malnourished rats. Int J Biol Chem 2010; 4: 10-18.

Alada ARA. The haematological effect of Telfairiaoccidentalis diet preparation. Afr J Biomed Res 2000; 3: 185-186.

Akubue PI, Kar A, Nnachetta FN. Toxicity of extracts of roots and leaves of Telfairiaoccidentalis. Planta Med 1980; 38: 339-343.

Fasuyi AO. Nutritional potentials of some tropical vegetable leaf meals: chemical characterization and functional properties. Afr J Biotech 2006; 5: 49-53.

Obeagu EI, Chikelu IM, Obarezi TN, Ogbuabor BN, Anaebo QBN. Haematological Effects of Fluted Pumpkin (Telfairiaoccidentalis) Leaves in Rats. Int J Life Sci Biotech Pharma Res 2014; 3(1): 172-182.

Salman TM, Olayaki LA, Oyeyemi WA. Aqueous extract of Telfairiaoccidentalis leaves reduces blood sugar and increases haematological and reproductive indices in male rats. Afr J Biotech 2008; 7(14): 2299–2303.

NIH. Guide for Care and Use of Laboratory Animals. 11th Edition. Washington, DC: National Academics Press; 2011: 43-48.

Dacie JV, and Lewis SM. Practical Haematology. Churchill Living-stone, New York: 1991.

Norusis MJ. Statistical Packages for Social Sciences (SPSS). SPSS Inc. SPSS/PC+ for Windows, base and advanced statistics users guide, Version 15.0 Chicago, IL: 1998.

Kayode, OT, Kayode AAA, Odetola AA. Therapeutic effect of Telfairia occidentalis on protein-energy malnutrition- induced liver damage. Res J Med Plant 2009; 3: 80-92.

Ekpenyong CE, Akpan EE, Udoh NS. Phytochemistry and Toxicity Studies of Telfairia occidentalis Aqueous Leaves Extract on Liver Biochemical Indices in Wistar Rats. Am J Med Med Sci 2012; 2(5): 103-110.

Oboh G. Hepatoprotective property of ethanolic and aqueous extracts of Telfairia occidentalis (Fluted pumpkin) leaves against gastric – induced oxidative stress. J Med Food 2005; 8: 560-563.

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