Antibacterial activity of crude extracts of the leaves of Nauclea latifolia Smith (Rubiaceae) and some selected conventional antibiotics on clinical isolates of Salmonella typhi
DOI:
https://doi.org/10.30442/ahr.0603-02-88Keywords:
Antibiotics, Antimicrobial activity, Nauclea latifolia, Salmonella typhi, Typhoid feverAbstract
Background: Nauclea latifolia Smith (Rubiaceae) is a valuable medicinal plant used in folkloric medicine in the treatment of Typhoid fever. The public health significance of Salmonella typhi, the aetiologic agent in typhoid fever lies in the increasing resistance to antimicrobial agents.
Objectives: To determine the antimicrobial potentials of the crude extracts of the leaves of Nauclea latifolia on clinical isolates of Salmonella typhi compared with some conventional antibiotics.
Methods: Antibiogram was carried out on 25 biochemically confirmed clinical isolates of Salmonella typhi using disc diffusion method of Kirby Bauer. Nauclea latifolia leaves extracted with ethyl acetate and methanol in a Soxhlet apparatus were screened for secondary metabolites. The minimum inhibitory concentrations (MICs) of the extracts were determined by agar dilution method on the isolates.
Results: The antibiogram of Samonella typhi showed the following resistance patterns: Augmentin® (24.0%), amoxicillin (44.0%), chloramphenicol (32.0%), gentamicin (20.0%), cloxacillin (96.0%), ciprofloxacin (8.0%), erythromycin (60.0%), tetracycline (32.0%) and cotrimoxazole (60.0%). The extracts showed good antibacterial activity on the clinical isolates including those resistant to antibiotics. The MIC of methanol extract ranged from
1.56mg/ml to 6.25mg/ml and 3.13mg/ml to 25.0 mg/ml for ethyl acetate extract with exception of isolates 11, 12, 19 and 20 with high MIC values as 50mg/ml. The methanol and ethyl acetate extracts gave a yield of 11.7% and 3.5% respectively. Bioactive secondary metabolites were detected from the extracts.
Conclusion: The crude extracts of the leaves of Nauclea latifolia contained antimicrobial agents that were active on Salmonella typhi which could be used alone and in supportive with conventional antibiotics for therapeutic management of typhoid fever.
References
Duke JA. Ethnobotanical uses of Nauclea latifolia. Phytochemical and Ethnobotanical 2008: Databases.
Adeleke OE, Adepoju TJ, Ojo AA. Prevalence of typhoid fever and antibiotic susceptibility pattern of its causative agent, Salmonella typhi. Niger J Microbiol 2006; 20: 1191-1197.
Crump JA, Krestinger K, Gay K, Hoekstra RM, Vugia DJ, Hurd S,.et al. Clinical response and outcome of infection with Salmonella enterica serotype Typhi with decreased susceptibility to fluoroquinolones. Antimicrob Agent Chemother 2008; 52: 1278-1284
Reddy KR, Rajesh PKM, Sekar U. Antibiotic susceptibility pattern and plasmid profile of multidrug-resistant Salmonella typhi. Indian J Med Microbiol 2005; 23: 208.
Akinyemi KO, Smith ST, Oyefolu AO, Coker AO. Multidrug-resistance in Salmonella entericaserovar Typhi isolated from patients with typhoid fever complications in Lagos, Nigeria. Public Health 2005; 119: 321-327.
WHO. Traditional medicine and modern health care progress reported by the Director-General Document No A44110 WHO Geneva.1991.
Rukangira E. The African Herbal Industry and Challenges. www.conserveafrica.org 2000.
Clinical and Laboratory Standard Institute, (CLSI) Performance standard for antimicrobial disk susceptibility tests 2009 (M02-A10). Wayne, PA: CLSI
Harbone JB. Phytochemical methods, a guide to modern techniques of plant analysis. 3rd edition, Chapmann and Hall. London, UK. 1998; p.278.
Gautam V, Gupta NK, Chaudhary U, Arora DR. Sensitivity pattern of Salmonella serotypes in Northern India. Braz J Infect Dis 2002; 6: 6.
Bhutta ZA, Khan IA, Shadmani M. Failure of short-course ceftriaxone chemotherapy for multidrug-resistant typhoid fever in children: a randomized controlled trial in Pakistan. Antimicrob Agents Chemother 2000; 44: 450-452.
Wallace JR. Antimicrobial properties of plant secondary metabolites. Proc Nutr Soc 2004; 63: 621-629.
Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000; 31: 247-256.
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